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时间：2006-01-01 23:05:20 编辑加入/取消收藏订制/取消短消息举报该贴

题目：manganese complexes of curcumin analogues:evaluation of hydroxyl radical scavenging ability,superoxide dismutase activity and stability toward hydrolysis
作者：Vajragupta.O;Boonchoong.P;Berliner.L
期刊：Free Radic Res 38:303~314;2004
谢谢。我的EMail:huali686@163.com

回复人：benchi,▲ (向大家学习) 时间：2006-01-02 04:35:44 编辑	1楼
用中文啊

回复人：huali686,▲ () 时间：2006-01-02 09:09:03 编辑	2楼
它是洋鬼子的文章

回复人：wenchunlin,▲▲▲ (欢迎光临我的论坛http://www.happychem.com.cn/bbs) 时间：2006-01-02 10:20:25 编辑

3楼

Abstract:

In order to improve the antioxidant property of curcumin and its analogue, diacetylcurcumin, manganese was incorporated into the structures in order to enhance superoxide dismutase (SOD) activity. Manganese (Mn) complexes of curcumin (CpCpx) and diacetylcurcumin (AcylCpCpx) were synthesized and firstly investigated for SOD activity and hydroxyl radical (HO•) scavenging ability. SOD activity was evaluated by both the nitroblue tetrazolium (NBT) reduction assay and electron paramagnetic resonance (EPR) with 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) as a spin trapping agent. CpCpx and AcylCpCpx inhibited the NBT reduction and decreased the DMPO/OOH adduct much greater than corresponding antioxidants or ligands, with IC<PRE>50</PRE> values of 29.9 and 24.7 M (NBT), and 1.09 and 2.40 mM (EPR), respectively. For EPR, potassium superoxide (KO<PRE>2</PRE>) was used as a source of O2-• where qualitative results suggested that CpCpx and AcylCpCpx were SOD mimics, which catalyze the conversion of O2-• to dioxygen and hydrogen peroxide (H<PRE>2</PRE>O<PRE>2</PRE>). Additionally, CpCpx and AcylCpCpx exhibited the great inhibition of DMPO/OH adduct formation with an IC<PRE>50</PRE> of 0.57 and 0.37 mM, respectively, which were comparable to that of curcumin (IC<PRE>50</PRE> of 0.64 mM), indicating that both Mn complexes are also an effective HO• scavenger. The stability against hydrolysis in water, various buffers and human blood/serum was carried out in vitro. It was found that both Mn complexes were pH and salt concentration dependent, being more stable in basic pH. In the human blood/serum test, CpCpx was more stable against hydrolysis than AcylCpCpx with about 10 and 20% of free Mn<PRE>2+</PRE>releasing, respectively.

回复人：wenchunlin,▲▲▲ (欢迎光临我的论坛http://www.happychem.com.cn/bbs) 时间：2006-01-02 10:21:32 编辑	4楼
连接 http://www.ingentaconnect.com/content/tandf/gfrr/2004/00000038/00000003/art00010

回复人：erro,★ (药物合成…… 希望认识更多的朋友) 时间：2006-01-02 11:38:00 编辑	5楼
还是我来吧 http://free.ys168.com/?erro huali686 哈哈!

回复人：huali686,▲ () 时间：2006-01-02 19:53:27 编辑	6楼
已经打分了。

回复人：huali686,▲ () 时间：2006-01-02 19:54:26 编辑	7楼
谢谢各位！

得分人：erro-3,

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