问题：求助:基质金属蛋白酶抑制剂GM6001(Ilomastat)比旋度的文献值
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时间：2006-03-09 17:37:00 编辑加入/取消收藏订制/取消短消息举报该贴

请教大家如何查找基质金属蛋白酶抑制剂GM6001(Ilomastat)比旋度的文献值

回复人：沧海一声笑,★★★ (为国家富强奋斗，为人民疾苦呐喊！) 时间：2006-03-09 21:22:35 编辑

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希望这个有用.后面的文献可能会提到:
https://www.elastin.com/newcatalog/browse.asp?cat=6007&path=6,6007
GM6001, Matrix Metalloprotease Inhibitor, 5mg

GM6001, ilomastat, Galardin?/P>Molecular Formula: C20H28N4O4
FW 388.47

Prepared by the method of Grobelny et al (1).

Inhibits a wide varietyof matrix metalloproteases with Ki抯 in the nanomolar range (1, 2).

Active in a number of animal models of diseases where matrixmetalloproteases are thought to be involved (2, 3, 4, 5, 6).

Purity is greater than 95 % as determined by HPLC.
Ki 1 nM or less against humanskin fibroblast collagenase, human 72 kd gelatinase, human 92 kdgelatinase and human neutrophil collagenase, Ki 27 nM against humanstromelysin (2).

References:
(1.)Grobelny, D., Poncz, L. and Galardy, R. E. 1992. Inhibition of Human Skin Fibroblast Collagenase, Thermolysin, and PseudomonasAeruginosa Elastase by Peptide Hydroxamic Acids. Biochemistry 31:7152-7154.

(2.) Galardy, R.E., M. E.Casabonne, C. Giese, J.H. Gilbert, F. Lapierre,H. Lopez, M.E. Schaefer, R. Stack, M. Sullivan, B. Summers, R. T. D.Tyrrell, J. Wee, S.D. Allen J.J. Castellot, J.P. Barletta, G.S. Schultz,L.A. Fernandez, S. Fisher, T.-.Y Cui, H.G. Foell-mer, D. Grobelny, andW.M. Holle-ran 1994. Low Molecular Weight Inhibitors in CornealUlceration, Ann NY Acad. Sci. 732: 315-323.

(3.) Bendeck, M.P., Zempo. N., Clowes, A.W., Galardy, R.E., and Reidy,M.A.1994. Smooth Muscle Cell Migration and Matrix MetalloproteaseExpression After Arterial Injury in the Rat. Circ. Res. 75: 539-545.

(4.) Gijbels, K., Galardy, R.E., and Steinman, L. 1994. Reversal ofExperimental Autoimmune Encephalomyelitis with a Hydroxamate Inhibitorof Matrix Metalloproteases. J. Clin. Invest. 94: 2177-2182.

(5.) Wang, P., Ba, Z.F., Galardy, R.E., and Chaudry. I.H. 1996.Administration of a Matrix Metalloproteinase Inhibitor after HemorrhageImproves Cardiovascular and Hepatocellular Function. Shock 6: 377-382.

(6.) Solorzano, C.C., Ksontini, R., Pruitt, J.H., Auffenberg, T.,Tannahill C., Galardy, R.E., Schultz, G.P., MacKay, S.L.D., Copeland,E.M., and Moldawer, L.L. 1997. A Matrix Metalloproteinase InhibitorPrevents Processing of Tumor Necrosis Factor a (TNFa) and Abrogates Endotoxin-Induced Lethality. Schock 7:427-431

http://www.biocompare.com/productdetails/177136/item/3236/matrix/GM6001-MMP-Inhibitor-from-CHEMICON.html
General Information
Item GM6001 MMP Inhibitor
Company CHEMICON
Price
Catalog Number CC1000
Size 1 mg
Request Information
More Information Company Product Page
Product Description
GM6001 MMP inhibitor, also known as Ilomastat or N-[(2R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl]-L-tryptophan methylamide, is a potent inhibitor of collagenases. Molecular weight: 388.47 Reported Ki values are as follows (see Galardy Ref):· Human MMP-1 (Fibroblast collagenase): 0.4 nM· Human MMP-3 (Stromelysin): 27 nM· Human MMP-2 (72 kDa gelatinase): 0.5 nM· Human MMP-8 (Neutrophil collagenase): 0.1 nM· Human MMP-9 (92 kDa gelatinase): 0.2 nM Typical working dilution for use in cultured cells is 10-25 µM. GM6001 will precipitate from aqueous solutions exceeding 100µM. Aqueous dilutions should be performed using low salt buffers to prevent precipitation of GM6001 from solution (Recommended dilution buffer: 50 mM Tris pH7.5, 150 mM NaCl, and 20 mM CaCl2.2H20). It is recommended that the concentration of GM6001 in aqueous working solutions is determined upon dilution and confirmed prior to each use. Relative concentrations of GM6001 in aqueous solutions may be determined by measuring the absorbance of the solution (tyrosine) at 280 nm.
CHEMICON Contact Information (More information about CHEMICON)
CHEMICON
CHEMICON International, Inc.
28820 Single Oak Drive
Temecula, CA 92590
Customer Service: 800-437-7500
Tech Support: 800-437-7500
Fax Number: 800-437-7502
Web Site: http://www.chemicon.com

More information about CHEMICON

回复人：HANHAN0630,▲ () 时间：2006-03-10 10:18:11 编辑	2楼
非常感谢,非常感谢.

得分人：沧海一声笑-1,

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