问题：硒代蛋氨酸定量分析方法
类型：求助 (悬赏分:3分)
提问：chsxchemis
等级：▲▲▲▲▲
版块：分析化学(wangsui,maqinghe,peiliang,)
信誉：97%
回复：4
阅读：1109
时间：2006-06-12 11:51:19 编辑加入/取消收藏订制/取消短消息举报该贴

请教高人硒代蛋氨酸定量分析方法，谢谢！

回复人：celan,▲▲▲▲ (化学品成分和结构剖析服务:www.chemnmr.com) 时间：2006-06-14 07:18:24 编辑

1楼

Hybridation of different chiral separation techniques with ICP-MS detection for the separation and determination of selenomethionine enantiomers: chiral speciation of selenized yeast.
Biomed Chromatogr. 2001 May;15(3):181-8.
Enantioseparation and determination of selenomethionine enantiomers in selenized yeast was investigated using chiral separation techniques based on different principles, coupled on-line to inductively coupled plasma mass spectrometry (ICP-MS) for selenium-specific detection. High performance liquid chromatography (HPLC) on a beta-cyclodestrin (beta-CD) column, cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC), gas chromatography (GC) on a Chirasil-L-Val column, and HPLC on a Chirobiotic T column have been investigated as the chiral separation techniques. For HPLC separation on the beta-CD column, and also for CD-MEKC, selenomethionine enantiomers were derivatized with NDA/CN(-). For chiral separation by GC, selenomethionine enantiomers were converted into their N-trifluoroacetyl (TFA)-O-alkyl esters. The developed hybridation methodologies are compared with respect to enantioselectivity, sensitivity and analysis time. The usefulness of the best-suited method [HPLC (Chirobiotic T)-ICP-MS] was demonstrated by its application to the successful chiral speciation of selenium and D-and L-selenomethionine content determination in selenized yeast. Copyright 2001 John Wiley & Sons, Ltd.

回复人：celan,▲▲▲▲ (化学品成分和结构剖析服务:www.chemnmr.com) 时间：2006-06-14 07:20:14 编辑	2楼
selenomethionine硒代甲硫氨酸手性分析HPLC ［格式： ] http://www.chem17.com/company/bang_article.asp?c_id=1287

回复人：celan,▲▲▲▲ (化学品成分和结构剖析服务:www.chemnmr.com) 时间：2006-06-14 07:21:11 编辑	3楼
Determination of amino acids in Selenium-enriched Spirulina platensis by reversed phase high performance liquid chromatography http://mirror.switch.ch/mdpi/cji/cji/2005/074032pe.htm

回复人：chsxchemis,▲▲▲▲▲ () 时间：2006-06-19 17:38:39 编辑	4楼
thank you!

得分人：celan-2,

问题讨论没有结束...

您尚未进入本论坛,登录之后才可以回贴

7msec