问题：生物化工问题
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提问：sahala1017
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版块：一般性化工问题(qixie,dingshx,wangnc3,)
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时间：2009-09-25 19:39:34 编辑加入/取消收藏订制/取消短消息举报该贴

请问哪位知道甲酸脱氢酶的结构式是怎样的？它催化反应的时候活性中心是哪里？

回复人：claymore,★★★★★ (C-H活化，kumada,suzuki,stille,negishi.cross-coupling.) 时间：2009-09-26 06:41:32 编辑

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Crystal Structure of Formate Dehydrogenase H: Catalysis Involving Mo, Molybdopterin, Selenocysteine, and an Fe4S4 Cluster
Science 28 February 1997:
Vol. 275. no. 5304, pp. 1305 - 1308

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Formate dehydrogenases
are a set of enzymes that catalyse the oxidation of formate to bicarbonate, donating the electrons to a second substrate, such as NAD+ in formate:NAD+ oxidoreductase (EC 1.2.1.2) or to a cytochrome in formate:ferricytochrome-b1 oxidoreductase

NAD-dependent reaction：
Formate + NAD(+) <=> CO(2) + NADH

Cytochrome-dependent reaction：
Formate + 2 ferricytochrome b1 <=> CO(2) + 2 ferrocytochrome b1 + 2 H(+)

References
1.Ferry JG (1990). "Formate dehydrogenase". FEMS Microbiol. Rev. 7 (3-4): 377–82.
2.Popov VO, Lamzin VS (1994). "NAD(+)-dependent formate dehydrogenase". Biochem. J. 301 ( Pt 3): 625–43.
3. Jormakka M, Byrne B, Iwata S (2003). "Formate dehydrogenase--a versatile enzyme in changing environments". Curr. Opin. Struct. Biol. 13 (4): 418–23.
4. Graham A, Boxer DH (1981). "The organization of formate dehydrogenase in the cytoplasmic membrane of Escherichia coli". Biochem. J. 195 (3): 627–37.
5.Ruiz-Herrera J, DeMoss JA (1969). "Nitrate reductase complex of Escherichia coli K-12: participation of specific formate dehydrogenase and cytochrome b1 components in nitrate reduction". J. Bacteriol. 99 (3): 720–9.

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